CD25 anticorps

N° du produit ABIN120428

L’anticorps Souris Monoclonal anti-CD25 a été validé pour FACS, IP, IHC (fro), IHC (p) et EIA. Il convient pour détecter CD25 dans des échantillons de Rat.

Aperçu rapide pour CD25 anticorps (ABIN120428)

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD25 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: OX-39

Détail du produit anti-CD25 anticorps

Specificité: This antibody recognizes the alpha chain of CD25, otherwise known as IL-2 receptor alpha. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts. We recommend the use of SM265LE for this purpose.

Purification: Affinity chromatography on Protein G

Immunogène: Stimulated Rat T cells. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/1 mouse myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA. Immunoprecipitation. Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CD25

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: CD25 / IL2RA

Sujet: CD25 is a 55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

ID gène: 25704

NCBI Accession: NP_037295

UniProt: P26897

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation